Supplementary Materialsmolecules-24-04569-s001. actions of puerarin (1) (Physique 1), a major isoflavonoid from var. = 8C12). ** < 0.001 vs. the vehicle-treated OVX group. # < 0.05, ## < 0.001 vs. the sham-operated group. To exclude false-positive results, a Y-maze task was also performed, to evaluate the locomotor activity. Results Rabbit Polyclonal to ELOA1 showed that neither 17-estradiol (2) nor puerarin (1) altered the locomotor activity of OVX mice (Physique 3). Open in a separate window Physique 3 The effect Bambuterol HCl of puerarin (1) and 17-estradiol (2) around the locomotor activity in the Y-maze task. The number of arm entries of each group was decided. Each column represents the mean SEM (= 8C10 in each animal group). 2.2. Changes in Uterine Weights and Volumes after the Administration of Puerarin (= 8C9). ## < 0.001 vs. the sham-operated group. * 0.05, ** < 0.001 vs. the vehicle-treated OVX group. ? < 0.05 between treatments with different doses. 2.3. Administration of Puerarin (< 0.001 vs. the sham-operated group. * < 0.05 vs. the vehicle-treated OVX group. 2.4. Effects of Puerarin (= 3C5). ## < 0.001 vs. the sham-operated group. * < 0.05 and ** < 0.001 vs. the vehicle-treated OVX group. 2.5. Puerarin (< 0.001 vs. the sham-operated group. ** < 0.01 vs. the vehicle-treated OVX group. ?? < 0.001 between treatments with different doses (for detailed statistical analysis, see Supplementary Materials, Table S9). 3. Conversation The present study aimed to investigate the effects of puerarin (1), a major constituent of a Thai medicinal herb var. (ppm), using residual solvent peaks (DMSO-d6) as recommendations. FABMS data Bambuterol HCl were obtained by using a JEOL JMS 700 mass spectrometer (JEOL Ltd., Tokyo, Japan) and var. (Airy Shaw & Suvat.) Niyomdham (Family Fabaceae) was collected in Ubon Ratchathani Province, Northeastern Thailand, in March 2010. The herb material was recognized by Dr. Thaweesak Juengwatanatrakul, Faculty of Pharmaceutical Sciences, Ubon Ratchathani University or college, Ubon Ratchathani Province, Thailand. A voucher specimen (NI-PSKKU 007C010) was deposited at the Herbarium of the Faculty of Pharmaceutical Sciences, Khon Kaen University or college, Thailand. The dried powdered bark (10 kg) was extracted with hexane (3 45 L) and filtered with Whatman No. 1 filter paper. The dried residue was extracted with EtOAc (3 60 L), and the process was repeated with EtOH (3 60 L). The EtOH extracts were combined and evaporated under reduced pressure, to give 226 g of a crude ethanol extract, which was subsequently dissolved in H2O (450 mL), with the assistance of an ultrasonic bath. The aqueous answer of the extract was applied on a column of Diaion HP-20 (2.26 kg, Mitsubishi Chemical Corp, Tokyo, Japan) and eluted with H2O (20 2 L), MeOHCH2O [(1:1), 15 2 L], MeOHCH2O [(3:1, 10 2 L)], MeOH (8 2 L), and EtOAc (5 2 L). The fractions eluted MeOHCH2O (1:1) were combined and evaporated under reduced pressure, to give a crude aqueous methanol extract (55.3 g), which was applied over a column of silica gel (1.1 kg) and eluted with mixtures of Bambuterol HCl CHCl3CMeOH and CHCl3CMeOHCH2O, wherein 250 mL fractions were collected as follow: Fractions (Frs.) 1C3 (CHCl3CMeOH, 9:1), 4C20 (CHCl3CMeOH, 17:3), 21C25 (CHCl3CMeOH, 4:1), 26C54 (CHCl3CMeOHCH2O, 4:1:0.1), 55C63 (CHCl3CMeOHCH2O, 6.5:3.5:0.1), 64C68 (CHCl3CMeOH, 1:1), and 69 (MeOH). Frs. 37C42 were combined (15.53 g) and purified by reversed-phase high-performance liquid chromatography (HPLC) of the JASCO model 887-PU pump and a 875 UV variable-wavelength detector (JASCO International Co. Ltd., Tokyo, Japan), using TSKgel ODS-80TS column (5 um, 6 i.d. 60 cm 2, Tosoh Chemical substances Co. Ltd., Tokyo, Japan) with an isocratic elution Bambuterol HCl utilizing a combination of MeCNCH2O (3:17, < 0.05 were considered significant. SigmaStat? edition 3.5 (SYSTAT Software Inc., Richmond, CA, USA) was employed for statistical analyses. 5. Conclusions Today's research provides corroborative proof, for the very first time, that puerarin (1), a glycosyl isoflavone isolated from the main bark from the Thai medicinal seed var. var..