Data CitationsReddy PP, Gulyani A, Das R. of phosphorylated and total ERK levels in HEK293T cells. elife-50571-fig3-figsupp5-data1.xlsx (10K) GUID:?F3B3F2E6-F954-491A-8407-310773BBE326 Figure 3figure product 5source data 2: Quantification of phosphorylated and total ERK levels being a function Silodosin (Rapaflo) of Fyn expression in HEK293T cells. elife-50571-fig3-figsupp5-data2.xlsx (9.9K) GUID:?54C365E2-F5A8-4A52-9E23-282CB957CB75 Figure 3figure supplement 6source data 1: Quantification of degrees of total and active Fyn in U2OS cells. elife-50571-fig3-figsupp6-data1.xlsx (10K) GUID:?9A8079FB-EA52-4A29-9A0B-D7D7B34BCCD7 Figure 3figure supplement 7source data 1: Quantification of cell-perimeter and area in expressing cells. elife-50571-fig3-figsupp7-data1.xlsx (11K) GUID:?80498EA3-593C-4C7A-B99F-32779A5D5419 Figure 3figure supplement 9source data 1: Quantification of tagged Fyn expression levels in Fyn-KD HEK 293 T cells in accordance Silodosin (Rapaflo) with endogenous kinase levels in charge cells. elife-50571-fig3-figsupp9-data1.xlsx (9.9K) GUID:?910D6384-DA3E-478E-91CC-F9F33BCD2BA9 Figure 3figure supplement 10source data 1: Quantification of phophorylated and total ERK levels in expressing U2OS cells. elife-50571-fig3-figsupp10-data1.xlsx (10K) GUID:?225B4D98-A22A-474E-B592-A47E5B355CB7 Figure 3figure supplement 11source Silodosin (Rapaflo) data 1: Quantification of phosphorylated and total ERK levels in expressing C2C12 cells. elife-50571-fig3-figsupp11-data1.xlsx (10K) GUID:?BB9973E7-986F-42E8-9F2B-27295D4B862C Body 4source data 1: Light dosage-dependence of donor fluorescence recovery in acceptor photo-bleaching test out F29 biosensor. elife-50571-fig4-data1.xlsx (13K) GUID:?CEE0731E-A424-4071-9389-BD250E6F13D0 Figure 4source data 2: Light dosage-dependence of donor fluorescence recovery in acceptor photo-bleaching test out F29 biosensor and nonbinding mutant. elife-50571-fig4-data2.xlsx (12K) GUID:?BECE240A-F044-465D-9EC4-7E605CACCD7E Body 4figure supplement 1source data 1: Quantification of donor fluorescence recovery following acceptor photo-bleaching in cells treated with SFK inhibitor. elife-50571-fig4-figsupp1-data1.xlsx (19K) GUID:?87DE31D8-0ED6-430D-93AF-5D4CA03C27AB Body 5source data 1: Quantification of FRET amounts in low and high activity areas in U2Operating-system cells. elife-50571-fig5-data1.xlsx (11K) GUID:?40904110-31F0-45FB-A185-1919B592A39E Body 5source data 2: Quantification of nonbinding control FRET levels in low Prkwnk1 and high activity areas in U2OS cells. elife-50571-fig5-data2.xlsx (10K) GUID:?3E4FA4CC-FE7C-4D8E-8632-1F2CED503EEF Body 5source data 3: Quantification of FRET levels Silodosin (Rapaflo) in cells in treated with FAK inhibitor. elife-50571-fig5-data3.xlsx (18K) GUID:?9B83BCC1-6837-4840-891A-01FACCACCB44 Body 5figure dietary supplement 1source data 1: Quantification of F29 nonbinding mutant FRET amounts across cell areas. elife-50571-fig5-figsupp1-data1.xlsx (9.7K) GUID:?A14226BE-5DBC-4F8F-A680-D84D27F5FC44 Body 5figure product 2source data 1: Quantification of and F29 localization levels across cell. elife-50571-fig5-figsupp2-data1.xlsx (18K) GUID:?E30A6A03-43A2-4003-8831-BCF250FCB58A Number 5figure supplement 3source data 1: Quantification of donor-normalized FRET levels in low and high activity zones. elife-50571-fig5-figsupp3-data1.xlsx (11K) GUID:?796AE93F-B43F-4F24-AE5E-E4E61AE0968B Number 5figure product 3source data 2: Assessment of FRETT levels and Fyn kinase localization at different time points. elife-50571-fig5-figsupp3-data2.xlsx (11K) GUID:?F36E0836-188D-4FC0-9C57-F5E087D71492 Number 5figure product 4source data 1: Quantification of FRETT levels and kinase localization across determined cellular zones. elife-50571-fig5-figsupp4-data1.xlsx (10K) GUID:?AD10B7D7-EBD0-44AE-8FC3-13E1C8F5DB6A Number 6source data 1: Quantification of FRET levels in low- and high-activity zones in C2C12 cells. elife-50571-fig6-data1.xlsx (10K) GUID:?E646567B-1497-4F47-9140-DEC771242622 Number 6source data 2: Silodosin (Rapaflo) Quantification of FRET levels in low- and high-activity zones in Fyn-KD HEK293T cells. elife-50571-fig6-data2.xlsx (10K) GUID:?4667603E-02CC-4E88-ADAA-0F5426102B6E Number 6source data 3: Quantification of expression of imaging experiments. elife-50571-fig6-figsupp2-data1.xlsx (11K) GUID:?2194C659-2A99-4AFE-BF15-80B0B14EA149 Figure 6figure supplement 2source data 2: Analysis of spatio-temporal Fyn activity patterns in U2OS cells expressing different levels of Fyn kinase. elife-50571-fig6-figsupp2-data2.xlsx (10K) GUID:?DB9B30FF-79E3-4EE5-A6C4-641E45D5860F Number 7source data 1: Analysis of temporal patterns of Fyn activity in U2OS cell. elife-50571-fig7-data1.xlsx (10K) GUID:?6035E362-197B-484C-98F8-1CDF915C784A Number 7source data 2: Analysis of temporal patterns of Fyn activity in C2C12 cell. elife-50571-fig7-data2.xlsx (10K) GUID:?5BB48794-9BF0-4196-8145-C4D80932A234 Number 7source data 3: Analysis of temporal patterns of Fyn activity in Fyn-KD HEK 293 T cell. elife-50571-fig7-data3.xlsx (10K) GUID:?C29E3A0F-CFC3-47E7-A937-0C92A84A3A07 Figure 7source data 4: Quantification of Fyn activity pulse time-period across different cell-types. elife-50571-fig7-data4.xlsx (10K) GUID:?44E90459-D240-47D5-9762-950A7C8AE207 Figure 7source data 5: Quantification of Fyn activity relative to distance from cell membrane in C2C12 cell. elife-50571-fig7-data5.xlsx (25K) GUID:?00BFF6B2-843A-4609-8E02-22748EC198C3 Number 7figure supplement 1source data 1: Quantification of FRETT signal over time in multiple U2OS cells. elife-50571-fig7-figsupp1-data1.xlsx (10K).